High-Oxidized Low Density Lipoprotein, Human

Cat No: H7980

Size: 2.0mg(milligrams)Protein/vial

Concentration: >1mg/ml protein （Based on actual label concentration）

Specifications: High Oxidized LDL is membrane filtered and aseptically packaged in a solution

containing phosphate-buffered saline at pH 7.4.

Storage:

This product is stable for only 3 weeks after receipt when handled aseptically and stored at 2-8°C (Do not freeze). After prolonged storage, some precipitate may be observed. This is normal for this product. Spin in a centrifugation 1000 x g for 3 minutes before using.

Biological Activity:

Sample lots of Oxidized LDL are evaluated for receptor binding to peritoneal macrophages in conjunction with our DiI-Ox-LDL and [I-125] Ox-LDL

Preparation:      

Human LDL (which was purified to homogeneity via ultra centrifugation(1.019-1.063g/cc)) is extensively oxidized with Cu₂SO₄ (oxidant) in PBS at 37°C. Oxidation is terminated by adding excess EDTA-Na₂.  Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of Ox-LDL migrates 3.0fold further than the native LDL. This product can produce potent oxidative stress and can be used to induce cell apoptosis/death (>50%) and produce cell injure model.

TBARS:

TBARS is determined colorimetrically by using Malondialdehyde as a standard.

Starting  LDL       0.5±0.5   nmoles of  MDA/mg Protein

High     Ox-LDL   90.0±9.9 nmoles of  MDA/mg Protein